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Saturday, November 30, 2013

Fawzy

Life Sciences, Vol. 64, No. 11, pp895-904, 1999 Scientific task: Characterizing the effects of dexamethasone on ODC gene expression and enzyme activity in the lung of tail pups. Facts Pertaining the Problem: Numerous studies have shown that gestational exposure to glucocorticoid increases the productions and twist of surfactant and accelerates morphological development of the fetal lung, while faulting of the pituitary-adrenal bloc violates lung growth, as do checkors of glucocorticoid biosynthesis. Synthetic glucocorticoids are routinely administered to large(predicate) women at risk of premature delivery in an fetch out to accelerate the functional maturation of the fetal lung and thereby issue the incidence of respiratory distress syndrome in the newborn, a star cause of neonatal death in pre-term infants. Glucocorticoids did not bowdlerize surfactant production when given postnatally. However, they were found to inhibit proliferation of lung electric cells and to impair formation of new alveoli, suggesting that endogenous glucocorticoids whitethorn modulate cell development in the postnatal lung. The Proposed provisional Solution: repayable to its extremely short half-life in animal tissues, ODC is speedily induced or suppressed by agents which influence development.
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The plan for the Experiment to Test the Hypothesis: wet-nurse CD female rats with litters of 10 pups were housed in breeding cages in a vivarium keep at 22 degrees Celsius with a 12-h light-dark cycle, and provided regimen and water ad libitum. Animals were killed by decapitation and lungs and livers were quickly dissected, homogenise in 19 volumes! of ice-cold 10mM Tris-HCl, centrifuged at 26,000 x g for 20 min, and the supported assayed for ODC activity by a change of the method of Russell and Synder. good liver RNA was extracted with acid guanidinium thiocyanate-phenol-choloform check to Chomczynsky and Sacchi. The Conclusion relate to the Objectives: The results from these studies demonstrate that neonatal lung ODC activity is highly sensitive... If you want to lead a full essay, order it on our website: OrderCustomPaper.com

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